Genetics

Retinoblastoma

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  • Tumour of primitive photoreceptor cells
  • Commonest ocular malignancy of childhood: 1 in 20,000
  • M=F
  • 40% are inherited: tumours tend to be bilateral and multicentric
  • Unilateral tumour is nearly always sporadic
  • Hereditary cases: only 10% have a positive family history. The remainder have a new mutation in the germ cells

    • The hereditary pattern is autosomal dominant but at the cellular level the condition behaves in a recessive manner

    • Hemizygosity of the Rb locus within band 13q14 confers a predisposition (germ line mutation carrier)

    • Rb locus is a member of the tumour suppressor gene class
    • At least 1 active normal allele is needed to prevent uncontrolled proliferation

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Familial cases of retinoblastoma also carry risk of osteosarcoma and pinealoma (so-called trilateral disease)

  • Genetics
    • Knudson’s two-hit hypothesis proposed two mutational events in retinoblastoma
      • First mutation in the germinal cell (therefore every cell) either by inheritance of germinal mutation, or by somatic mutation

      • Second mutation is somatic within an already mutated cell
      • Note: two somatic mutations would be needed in sporadic cases hence they are likely to be just unilateral

      • Examples: retinoblastoma, neurofibromatosis, BRCA1 and 2, tuberous sclerosis, Von-Hippel-Lindau, familial melanoma

      • Most genes responsible for dominantly inherited cancer syndromes are tumour suppressor genes because the second hit occurs after birth

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