Physiology

Muscle Physiology

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  • Presynaptic neurotransmitter release
    • Occurs via exocytosis
    • Controlled by calcium influx
    • Quantal 
  • Post-synaptic potentials spread through muscle via the transverse tubular system, an extension of the sarcolemma

    • Acetylcholine binds to postsynaptic receptors on the motor end-plate
    • This triggers sodium ion influx producing a depolarisation.
      • An excitatory action potential is produced when a threshold is reached and sodium channels are activated bringing about a massive influx of sodium ions

      • The density of sodium channels is highest at the initial segment of the axon (the “axon hillock” of motor neurones or the first node of Ranvier for myelinated sensory neurones).

      • The membrane potential reaches positive values, reaching a threshold triggering an action potential

      • Repolarisation later occurs due to a decrease in sodium conductance and slow increase in potassium conductance allowing potassium to leave the cell

    • The tubular system is a continuation of the sarcoplasmic reticulum and is a calcium ion reservoir

    • Action potential stimulates calcium release from this tubular system by opening DHP receptors

    • Calcium binds to troponin C associated with tropomyosin on the actin filament. 
      • A conformational change occurs to displace tropomyosin
    • The displacement of tropomyosin frees the myosin binding sites in the actin grooves
    • The myosin can combine with actin to form “cross-bridges”
    • Myosin magnesium-dependent ATPase generates energy which effects the movement of the myosin heads and muscle contraction

    • Calcium accumulates back in the SR via the action of ATPase
  • Striated muscle (and cardiac muscle): organised into units called sarcomeres
    • The region where two Z lines overlap
  • A band: overlapping myosin and actin
  • H band: myosin only
  • Skeletal muscle does not require an intrinsic pacemaker like cardiac muscle
  • Resting membrane potential is about -90mV: potassium contributes most to this
  • Muscle spindles
    • Intrafusal fibres
    • Innervated by type Ia, type II and gamma fibres
    • Respond to rate of change in muscle length
  • Action potential length
    • Cardiac: 200ms
    • Smooth: 50ms
    • Striated/skeletal: 10ms

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