Pharmacology

Pharmacokinetics

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  • Volume of distribution = dose/plasma concentration at time of administration
  • With repeated administration, the plasma concentration is a function of the rate of administration and the rate of elimination

    • Achieves steady state within 3-4 half-lives
    • Steady state can be achieved faster by using a loading dose
    • Drug half-life determines the frequency of subsequent doses
  • Bioavailability: the amount of oral dose that reaches the systemic circulation and is available to the site of action

    • May be limited by first-pass metabolism
      • Gut motility
      • Intestinal pH, bile salts
      • Enterohepatic circulation
      • Intestinal blood flow
      • Intestinal microflora

Hot Topic

Ocular topical drugs have higher bioavailability as they are absorbed via nasal mucosa and so avoid first-pass metabolism

  • Drug kinetics
    • First-order: linear increase in rate with increased drug concentration
    • Zero-order: saturation at high drug concentrations
      • Eg. when the capacity of liver enzymes is consumed
  • Drug penetration of cells
    • Diffusing through lipid (depends on lipid solubility)
    • Diffusion through aqueous pores
    • Via carrier molecules
    • Pinocytosis
  • Drug distribution
    • Binding affinity for plasma proteins eg. albumin
      • Highly bound drugs tend to remain in the plasma
    • Binding to tissue proteins
    • Blood flow to different tissues

Hot Topic

The blood-retinal and blood-aqueous barriers limit drug distribution in the eye. Usually only lipid-soluble drugs move between the blood and the retina. Systemically administered drugs do not necessarily produce high intraocular levels

  • Drug metabolism
    • Occurs almost entirely in the liver
    • Increases drug solubility for excretion
    • Might activate a prodrug to its active form
      • May inactivate others
    • Phase I: oxidation
      • Performed by cytochrome p450
      • This group of enzymes also exists in the eye
      • Numerous inducers and inhibitors (including chloramphenicol)
    • Phase II: conjugation
  • Drug excretion
    • Renal excretion: varies widely between drugs depending on the kidneys capacity to handle the drug’s properties

      • Glomerular filtration (if not protein-bound and small enough ie. <20,000Da)
      • Tubular secretion into the glomerular filtrate
      • Reabsorption
    • Biliary excretion
      • Conjugated drugs are concentrated in the bile and delivered to the intestine
      • Drug may be released and re-absorbed (enterohepatic circulation)

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