Genetics
Molecular Analysis
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Get access- DNA fragments are cut using restriction endonucleases
They recognise specific dsDNA sequences and cleave the sugar-phosphate backbone at these sites
- These sites tend to be palindromic
Any two fragments produced by a particular restriction endonuclease can be joined together
- Natural products of bacteria
DNA ligase catalyses the formation of phosphodiester bonds on each strand to create a recombinant DNA molecule
- They are then analysed according to their molecular weight by electrophoresis