Physiology

Fluid Balance

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  • 3 litres of plasma water, 11 litres of interstitial water, 14 litres of extracellular fluid and 28 litres of intracellular fluid

  • Total body water:
    • 60% intracellular
    • 40% extracellular
      • 20% intravascular
      • 73% interstitial
      • 7% transcellular (eg. CSF)
  • Plasma osmolality determines plasma osmotic pressure
    • Sodium accounts for 90% of osmolality but concentration is approximately equal between the intravascular and extravascular compartments

    • Sodium: 14 mmol/L intracellularly, 142 mmol/L extracellularly
    • Potassium: 140 mmol/L intracellularly; 4 mmol/L extracellularly
  • Interstitial fluid production is determined by
    • Plasma proteins exerting oncotic pressure
      • Albumin contributes 80%
      • Globulin contributes 20%
      • Fibrinogen contributes a small amount
    • Hydrostatic pressure
  • Renin-angiotensin aldosterone system
    • Angiotensin II is the final product of the pathway and causes
      • Vasoconstriction
      • Stimulation of aldosterone release 
      • Thirst
      • Increases plasminogen activator
      • Promotes microalbuminuria
    • Aldosterone (mineralocorticoid hormone)
      • Increases sodium reabsorption and potassium secretion via mineralocorticoid receptors (which are intracellular, nuclear receptors) in the distal tubule that upregulate expression of the basolateral Na/K pump

      • Upregulates ENaC receptors in the collecting ducts to further increase sodium reabsorption

      • Increases secretion of hydrogen ions via the intercalated cells of the tubules
      • Effects take approximately 1 hour and peak at 4 hours
      • Note also stimulates salt and water reabsorption in the gut, sweat and salivary glands

  • ADH (peptide hormone): released when hypertonic plasma stimulates hypothalamic osmoreceptors

    • Water retention via aquaporin expression in the collecting ducts
    • Vasoconstriction

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