Physiology

Oxygen-Haemoglobin Dissociation Curve

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  • Sigmoid shape: oxygen binding alters the protein shape to promote binding
  • Increased dissociation (curve moves right):
    • CO2 rise
    • Temperature rise
    • pH decreased (hydrogen ion rise)
    • Increased 2,3-DPG
  • Fetal haemoglobin has a higher affinity for oxygen: the curve lies left
  • Myoglobin has a higher affinity for oxygen cp. haemoglobin
  • Carbon monoxide has a very steep dissociation curve: can displace oxygen from haemoglobin
  • Methaemoglobin has no affinity for oxygen
  • Deoxygenated haemoglobin forms carbamino compounds more readily and so venous blood is more efficient at transporting carbon dioxide

    • 5% of CO2 is dissolved in plasma
    • 90% travels as bicarbonate
    • Carbonic anhydrase is found within red blood cells, not plasma

Haemoglobin

  • Derivative of porphyrin
  • Contains ferrous iron (Fe2+)
    • Oxidation of iron to ferric iron, Fe3+, occurs in methaemoglobinaemia
  • Globin: four polypeptide chains
    • HbA: two alpha chains and two beta chains

HbF: two alpha chains and two gamma chains (fetal Hb has a higher O2 affinity)

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